Fig. 6
From: Extensive identification and analysis of conserved small ORFs in animals
Experimental evidence supports translation of predicted sORFs and protein expression. a Translation is detected using the ORFscore method [10] on published ribosome profiling data. The Kolmogorov-Smirnov D-statistic is used to assess the performance of the dataset by comparing annotated sORFs to the negative control (dark gray). Length-matched non-conserved sORFs from non-coding transcriptome regions are included for comparison (light gray). ***P <0.001; **P <0.01; *P <0.05 (Mann-Whitney test). b Peptide expression of many predicted sORFs is confirmed by mining in house and published mass spectrometry datasets from cell lines and model organisms