Errors in RNA-Seq quantification affect genes of relevance to human disease

Table 4 Gene and MMG counts for MG994

	Lung cells tumour 1	Lung cells tumour 2	Lung cells tumour 3	Lung cells normal 1	Lung cells normal 2	Lung cells normal 3
ENSMUSG00000072674	0	0	0	0	0	0
ENSMUSG00000094800	0	0	0	0	0	0
ENSMUSG00000095304	0	0	0	0	0	0
MG994 (raw counts)	541	721	325	3298	3268	1471
MG994 (percentage total)	0.007	0.009	0.004	0.043	0.037	0.019

Counts data for MG994. The three genes included in MG994 have a read count of zero from the unique read analysis. However, MG994 has many reads that map uniquely within the group, and is differentially expressed between normal and tumour cells

ISSN: 1474-760X