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Table 1 Proportion of error-corrected SMRT reads containing cDNA primer, poly(A) tail, and canonical polyadenylation signal (AAUAAA). Low levels of the latter are expected, since RNA processing in plants generally shows a decreased dependence on the AAUAAA signal [36]

From: Exploiting single-molecule transcript sequencing for eukaryotic gene prediction

Dataset All With primer (%) With poly(A) (%) With primer and poly(A) (%) With poly(A) signal (%)
CCS 1-2 kb 36,143 92.2 64.3 60.9 21.2
CCS 2-3 kb 20,795 86.4 86.4 79.3 27.3
CCS 3 kb 22,027 86.0 89.1 81.9 29.9
Unmerged subreads 1-2 kb 181,522 13.4 28.5 28.5 9.7
Unmerged subreads 2-3 kb 223,925 10.2 33.5 33.5 11.6
Unmerged subreads 3 kb 221,424 10.6 36.2 36.2 12.6