Table 1 Proportion of error-corrected SMRT reads containing cDNA primer, poly(A) tail, and canonical polyadenylation signal (AAUAAA). Low levels of the latter are expected, since RNA processing in plants generally shows a decreased dependence on the AAUAAA signal [36]
From: Exploiting single-molecule transcript sequencing for eukaryotic gene prediction
Dataset | All | With primer (%) | With poly(A) (%) | With primer and poly(A) (%) | With poly(A) signal (%) |
|---|---|---|---|---|---|
CCS 1-2 kb | 36,143 | 92.2 | 64.3 | 60.9 | 21.2 |
CCS 2-3 kb | 20,795 | 86.4 | 86.4 | 79.3 | 27.3 |
CCS 3 kb | 22,027 | 86.0 | 89.1 | 81.9 | 29.9 |
Unmerged subreads 1-2 kb | 181,522 | 13.4 | 28.5 | 28.5 | 9.7 |
Unmerged subreads 2-3 kb | 223,925 | 10.2 | 33.5 | 33.5 | 11.6 |
Unmerged subreads 3 kb | 221,424 | 10.6 | 36.2 | 36.2 | 12.6 |