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Fig. 4 | Genome Biology

Fig. 4

From: Exploiting single-molecule transcript sequencing for eukaryotic gene prediction

Fig. 4

Gene model validation. An initial gene set was calculated based on the sugar beet reference genome (RefBeet-1.2) and publicly available gene expression data [5, 13] using AUGUSTUS default parameters. Genes from the initial gene set were validated using PacBio SMRT sequences and by manual curation. Additional gene models were determined solely from SMRT full-insert sequences. The latter were included to train the parameter set for the final BeetSet-2 gene prediction

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