Fig. 2

Role of KDM5B in the regulation of H3K4me3. a Tag density profile shows that KDM5B is recruited to promoters of expressed rather than silent genes in mouse embryonic stem cells. b Kdm5b knock-down results in increased H3K4me3 levels in the gene bodies and the corresponding loss of H3K4me3 at the TSSs of expressed genes. Box plot windows for the shaded areas are shown (right). * MWW p<1e-16. c Heatmaps show that Kdm5b knock-down causes spreading of H3K4me2 into the gene bodies, but not upstream, of the expressed genes. d Genomic distribution of H3K4me3 peaks. Top graphs show data for all peaks in control (shLuc)- and shKdm5b-expressing cells; bottom graphs show distribution for those areas where occupancy was significantly (p < 0.01, fold > 4) increased or decreased upon Kdm5b knockdown