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Fig. 7 | Genome Biology

Fig. 7

From: Estrogen-induced chromatin decondensation and nuclear re-organization linked to regional epigenetic regulation in breast cancer

Fig. 7

The effect of estrogen on chromatin compaction and nuclear organization at subregion 2 of the 16p11.2 RER region. a Map of the 16p11.2 RER subregion 2 showing the location of ER binding sites (red) in MCF7 cells (from [38]). Below, the location of genes in the region is shown from the UCSC Genome Browser NCBI36/hg18 assembly of the human genome. b, c Box plots comparing the distribution of normalized FISH interprobe distances (d 2 /r 2) measured across subregion 2 of the 16p11.2 RER region in ER+ MCF7 (b) and ER− MDAMB231 (c) breast cancer cell lines. Data are shown for cells grown in normal serum, in media stripped of hormone for 3 days (−E2), and after addition of 100 nm estrogen for 24 hours (+E2). n = 60 cells for each sample. d, e The percentage of FISH hybridization signals for subregion 2 of the 16p11.2 RER region found in each of five shells of equal area eroded from the edge of the nucleus (shell 1) through to the nuclear center (shell 5), in MCF7 (d) and MDAMB231 cells (e) grown in normal serum (grey shaded bars), hormone stripped media (−E2, white) and after addition of 100 nm estrogen for 24 hours (+E2, black)

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