Fig. 6

Automated determination of chromosome breaks and translocations by hiBA-FISH. a Jitter plot of the minimum Red/Green FISH per Red allele signal distances in the indicated cell lines and experimental conditions. ALK hiBA-ALK probe set, NPM hiBA-NPM1 set. One pixel = 320 nm. Each dot represents a measured Red/Green distance. The number of Red/Green distances plotted for each single experimental condition is indicated. Breakage events have a Red/Green minimum distance of more than four pixels (1.28 μm, red dashed line). b Crossbar plot indicates the frequency of cells with at least one breakage event (middle line) and its relative 95 % CI (from top to bottom line) expressed as percentages for the indicated cell lines and treatments as measured with the hiBA-ALK probe set for the datasets shown in (a). The same frequency values relative to Untreated/Mac2A cells were plotted in both the left and right panels for comparison purposes. c Same as (b), but for the hiBA-NPM1 set. d Jitter plot of the subset of Red FISH signals in proximity of a FarRed signal (Red/FarRed minimum distance ≤4 pixels). Translocation events have a Red/Green minimum distance of more than four pixels (red dashed line). Each dot represents a Red/Green distance. The number of Red/Green distances plotted for each single experimental condition is indicated. e Crossbar plot but for the frequency of cells with at least one NPM1-ALK translocation event as measured by the hiBA-ALK probe set. The same frequency values relative to Untreated/Mac2A cells are plotted in both the left and right panels for comparison purposes. f Same as (e), but for the hiBA-NPM1 probe set