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Fig. 2 | Genome Biology

Fig. 2

From: Quantitative detection of rare interphase chromosome breaks and translocations by high-throughput imaging

Fig. 2

Automated nucleus segmentation and FISH signal detection. a, c, e, g, i Maximal projections of 40× confocal image z-stacks of Mac2A cells stained with the hiBA-ALK probe set. The overlay represents a composite image of the Green (Alexa488), Red (Alexa568) and FarRed (Cy5) channels. The inset in each panel represents a magnified image of representative Mac2A cells. DAPI 4',6-diamidino-2-phenylindole. Scale bars 10 μm. b, d, f, h, j In silico generated images representing the detected nucleus ROI (yellow) and the FISH signal ROIs (in green, red and blue)

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Genome Biology

ISSN: 1474-760X

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