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Fig. 6 | Genome Biology

Fig. 6

From: Global SUMOylation on active chromatin is an acute heat stress response restricting transcription

Fig. 6

Positive correlation between promoter-bound Pol2, SUMO2/3 and PIAS1. a SUMO2/3, PIAS1, and Pol2 ChIP-seq tracks at HSP locus in VCaP cells in control (blue), HS (red), and recovery (orange) conditions. b Heat map showing SUMO2/3, PIAS1, and Pol2 ChIP-seq signals in control (C), HS, and recovery (Re) at TSS ± 1 kb window centered to annotated TSSs and arranged according to Pol2 signal in HS. Intensities are presented using false-color scale. Maximum signals for SUMO2/3, PIAS1, and Pol2 are 15, 5, and 40, respectively. c Promoter SUMO2/3 occupancy positively correlates with promoter Pol2 occupancy. Genes were classified to active (RPKM >0.2) and silent (RPKM ≤0.2) based on their promoter Pol2 signal. Promoter SUMO2/3 signal on active genes increased significantly from C via HS to Re. SUMO2/3 signal was below background level (gray line, median IgG ChIP-seq signal at promoters) at promoters of silent genes. Promoter SUMO2/3 signal increased with increasing promoter Pol2 signal when genes were divided into four categories of 4,051 genes based on their promoter Pol2 signal in control (blue), HS (red), or recovery (orange) conditions. d Promoter PIAS1 occupancy correlates with that of Pol2 only in HS. Gene categories are the same as in (c). e Immunoblotting with anti-Pol2 antibody shows that the presence of both hyperphosphorylated (Pol2 0) and non-phosphorylated (Pol2 A) in non-treated (CTRL) and vehicle-treated (DMSO) cells. DRB (100 μM, 3 h) efficiently inhibits hyperphosphorylation of Pol2 (Pol2 0), and triptolide (TRP; 1 μM, 3 h) leads to degradation of Pol2. Both DRB and TRP decrease the amount of high molecular mass SUMO2/3-modified proteins (anti-SUMO2/3). f DRB and TRP reduce the average promoter SUMO2/3 ChIP-seq signal

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