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Table 2 Phenotypic segregation analysis of T2 transgenic lines

From: Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation

T1 LTMs/Total-T2 T1 LTMs/Total-T2 T1 LTMs/Total-T2
1 20/20 (100 %) 9 0/83 (0) 17 11/34 (32.4 %)
2 0/162 (0) 10 33/156 (21.2 %) 18 0/42 (0)
3 20/20 (100 %) 11 27/49 (55.1 %) 19 0/53 (0)
4 42/98 (42.9 %) 12 0/78 (0) 20 0/36 (0)
5 15/47 (31.9 %) 13 0/52 (0) 21 18/45 (40.0 %)
6 25/77 (32.5 %) 14 0/202 (0) 22 n.a.
7 13/64 (20.3 %) 15 0/57 (0) 23 57/94 (60.6 %)
8 0/56 (0) 16 0/38 (0) 24 53/90 (58.9 %)
  1. LTMs, likely triple mutants, that is, T2 plants with phenotypes similar to those of try cpc etc2 triple mutants; Total-T2, total number of T2 plants examined; n.a., not available. The average segregation ratio of the LTMs to total T2 plants examined was 24.8 % ((100 % + 100 % + 42.9 % + … + 58.9 %)/24). The T2 seeds from #1 and #3 were sown on MS medium whereas those from the other T1 lines were sown on hygromycin (25 mg/L) medium