Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation

Table 2 Phenotypic segregation analysis of T2 transgenic lines

T1	LTMs/Total-T2	T1	LTMs/Total-T2	T1	LTMs/Total-T2
1	20/20 (100 %)	9	0/83 (0)	17	11/34 (32.4 %)
2	0/162 (0)	10	33/156 (21.2 %)	18	0/42 (0)
3	20/20 (100 %)	11	27/49 (55.1 %)	19	0/53 (0)
4	42/98 (42.9 %)	12	0/78 (0)	20	0/36 (0)
5	15/47 (31.9 %)	13	0/52 (0)	21	18/45 (40.0 %)
6	25/77 (32.5 %)	14	0/202 (0)	22	n.a.
7	13/64 (20.3 %)	15	0/57 (0)	23	57/94 (60.6 %)
8	0/56 (0)	16	0/38 (0)	24	53/90 (58.9 %)

LTMs, likely triple mutants, that is, T2 plants with phenotypes similar to those of try cpc etc2 triple mutants; Total-T2, total number of T2 plants examined; n.a., not available. The average segregation ratio of the LTMs to total T2 plants examined was 24.8 % ((100 % + 100 % + 42.9 % + … + 58.9 %)/24). The T2 seeds from #1 and #3 were sown on MS medium whereas those from the other T1 lines were sown on hygromycin (25 mg/L) medium

ISSN: 1474-760X