Line |
ETC2
|
TRY
|
CPC
| Genotype |
---|
T1 | #1 | +A/+C | −C/−C | +A/+T | eettcc |
T2 | 1-1 | +A/+C | −C/−C | +A/+T | eettcc |
1-2 | +A/+A | −C/−C | +A/+A | eettcc |
1-3 | +A/+C | −C/−C | +A/+T | eettcc |
1-4 | +A/+A | −C/−C | +A/+A | eettcc |
T1 | #3 | +T/+T | +T/+G | +G/+T | eettcc |
T2 | 3-1 | +T/+T | +G/+G | +G/+T | eettcc |
3-2 | +T/+T | +G/+G | +T/+T | eettcc |
3-3 | +T/+T | +G/+G | +G/+T | eettcc |
3-4 | +T/+T | +G/+G | +G/+T | eettcc |
T1 | #C1 | +C/+A | +T (×13)/−G (×11) | +G/+G | eettcc |
T2 | C1-17 | +A/+A | +T/+T | +G/+G | eettcc |
- All mutations but TRY from #C1 in this experiment were single-base insertions or deletions by direct sequencing of PCR products and the inserted (+) or deleted (−) nucleotide is denoted. TRY mutations in #C1 were detected by sequencing of cloned PCR products, and the number of the same type of mutation is indicated in parentheses. Two types of mutations from direct sequencing of PCR products were obtained based on double-peaks on chromatograph. Two alleles are separated by ‘/’. eettcc corresponds to etc2 try cpc triple mutant. C1-17 is a nontransgenic T2 line derived from #C1