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Table 1 Mutation analysis of three T1 likely triple mutants and their T2 progeny

From: Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation

Line ETC2 TRY CPC Genotype
T1 #1 +A/+C −C/−C +A/+T eettcc
T2 1-1 +A/+C −C/−C +A/+T eettcc
1-2 +A/+A −C/−C +A/+A eettcc
1-3 +A/+C −C/−C +A/+T eettcc
1-4 +A/+A −C/−C +A/+A eettcc
T1 #3 +T/+T +T/+G +G/+T eettcc
T2 3-1 +T/+T +G/+G +G/+T eettcc
3-2 +T/+T +G/+G +T/+T eettcc
3-3 +T/+T +G/+G +G/+T eettcc
3-4 +T/+T +G/+G +G/+T eettcc
T1 #C1 +C/+A +T (×13)/−G (×11) +G/+G eettcc
T2 C1-17 +A/+A +T/+T +G/+G eettcc
  1. All mutations but TRY from #C1 in this experiment were single-base insertions or deletions by direct sequencing of PCR products and the inserted (+) or deleted (−) nucleotide is denoted. TRY mutations in #C1 were detected by sequencing of cloned PCR products, and the number of the same type of mutation is indicated in parentheses. Two types of mutations from direct sequencing of PCR products were obtained based on double-peaks on chromatograph. Two alleles are separated by ‘/’. eettcc corresponds to etc2 try cpc triple mutant. C1-17 is a nontransgenic T2 line derived from #C1