Fig. 3

Structural and functional comparisons of twelve combinations of eight promoters and two terminators. a Seven combinations of EC1.1, EC1.2, or 2x35S promoters and rbcS E9 terminator (rbcS-E9t) or nos terminator (Nost). The pHEN2A-TRI and pHEN2C-TRI constructs have the same combination but different vector backbones: pGreen for the former and pCambia for the latter. The data for pHSN2A-TRI come from the publication and p2gR-TRI-A is renamed pHSN2A-TRI in this paper [26]. b Five combinations of five fusion promoters and the rbcS E9 terminator. Physical maps of the T-DNAs of seven (a) or five (b) CRISPR/Cas9 binary vectors are indicated. For each binary vector, the vector name, the promoter, the terminator, and the mutation frequencies of T1 transgenic plants are indicated at the same row under the maps. See Fig. 1 for RB/LB, zCas9, 2-sgRs, and Hyg. EC1p, EC1.1p or EC1.2p; 35Sen, CaMV 35S enhancer; EC1.2en, enhancer from EC1.2 promoter; LTM, likely triple mutant; Total, total number of T1 plants; Mosaics-I, type I mosaic plants with strong phenotypes indistinguishable from the double mutants; Mosaics-II, type II mosaic plants with the phenotypes appearing only in some parts of the whole plants. The ratios of T1 plants with the mutations (LTMs, Mosaics-I, or Mosaics-II) to total number of T1 plants are indicated