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Fig. 2 | Genome Biology

Fig. 2

From: Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation

Fig. 2

Phenotypic segregation of T2 transgenic lines. Phenotypic segregation of T2 transgenic lines derived from two representative T1 lines with normal phenotypes. Seeds from T0 plants were sown on MS medium containing 25 mg/L hygromycin, vernalized at 4 °C for 3 days, and grown under long-day conditions (16 h light/8 h dark) at 22 °C for 7 days. Hygromycin-resistant seedlings (T1) were transplanted to soil and allowed to grow for 20 days before photographing

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