Skip to main content

Table 1 Evaluation of total RNA and circRNA absolute quantity by ERCC spike-in RNAs

From: Single-cell RNA-seq transcriptome analysis of linear and circular RNAs in mouse preimplantation embryos

Stage

Oocyte

Zygote

2-cell

4-cell

8-cell

Morula

Blastocyst

Average number of mRNA molecules per embryo (×106)

26.8

21.3

4.5

5.7

6.6

13.8

21.1

Average number of circRNA molecules per embryo

2278

1850

1509

1422

1602

2799

779

Ratio of circRNA/host linear RNAR

1.6 %

1.7 %

2.2 %

4.7 %

4.2 %

6.8 %

9.8 %

Average number per type of circRNA transcript per embryo

71

64

24

15

21

41

158

Number of circRNA genesa per stage

363

425

684

517

433

474

123

Number of types of circRNA transcripts per stage

591

718

1,243

861

702

721

140

  1. The total mRNA copy number and circRNA copy number were evaluated through ERCC spike-in
  2. aCircRNA host genes; some host genes may generate several different types of circRNA transcripts