Fig. 6

Maternally and zygotically expressed genes in mouse preimplantation embryos. a Heatmap of maternally expressed genes. b The top enriched GO terms overrepresented in 1238 maternally expressed genes. c Heatmap of zygotically expressed genes. The zygotic genes are down-regulated after blocking of transcription with α-Amanitine treatment of the embryos. d The top enriched GO terms overrepresented in 4143 zygotically expressed genes. e Expression levels of 139 novel maternal genes and 57 novel zygotic genes in mouse oocytes, normal two-cell embryos and α-Amanitine treated two-cell embryos were analyzed. e (lLeft: the maternal genes show high expression levels in oocyte samples and are largely down-regulated in normal two-cell embryos. Some maternal genes are also still highly expressed in α-Amanitine treated two-cell embryos. (rRight: the zygotic genes are highly expressed in normal two-cell embryos. Out of the 57 novel zygotic genes, 54 could be validated, which means they are not transcribed in two-cell embryos treated with 100 ng/ml α-Amanitine. f RT-qPCR validation of both annotated and novel maternal and zygotic candidate genes. Maternal genes have ΔΔCt(two-cell-oocyte) > 1 and zygotic genes have ΔΔCt(two-cell-oocyte) < −1. Novel gene #1 to novel gene #4 refer to comp20337_c0, comp46577_c0, comp46858_c0 and comp89562_c0, respectively (also see Additional file 6)