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Fig. 2 | Genome Biology

Fig. 2

From: Comparative assessment of methods for the computational inference of transcript isoform abundance from RNA-seq data

Fig. 2

Influence of sequencing depth and expression levels on the accuracy of expression estimates. Transcript isoform and gene expression levels were estimated with each of the indicated methods from in silico-generated datasets of different ‘sequencing’ depths. The accuracy of a method was assessed in terms of the Spearman correlation coefficient (rs) between the estimates and the known input levels (‘ground truth’) of expressed transcripts (a) and genes (b). Based on their true abundances, transcripts (c) and genes (d) were distributed across four bins of expression levels. Estimation accuracies as in (a) and (b) are indicated for each method and bin. The numbers of transcripts and genes in each bin are indicated together with the expression ranges that they cover. Estimates are based on a sequencing depth of 30 million reads

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