Fig. 1

Mapping statistics of all samples and read lengths. a Each sample and read length is plotted with its respective percentage of uniquely mapped, multi-mapped and unmapped reads. The 25 bp reads have the lowest percent of uniquely mapped reads across all samples. Single-end reads also have higher percentage of multi-mapped reads and slightly lower percentage of uniquely mapped reads. b The number of splice junctions detected for each sample is plotted. The 25 bp samples detected the least number of junctions and single-end reads detected significantly fewer junctions overall than paired-end reads. The error bars represent the highest and lowest number of splice junctions detected across replicates