Fig. 5

Binding of the GFP-FOXM1 DBD mutants is significantly reduced genome-wide. a Heat map comparing binding events in WT FOXM1 and both FOXM1 DBD mutants. The window represents ± 5 kb regions centered on WT GFP-FOXM1 binding events with a second plot (on right) showing the average signal intensity of differential bound peaks. b Three examples of genomic regions showing binding peaks where binding is reduced in the GFP-FOXM1 DBD mutants compared to the WT. (c and d) Differential binding analysis (DBA) was used to identify significantly (FDR <0.05) differentially bound peaks in the WT GFP-FOXM1 compared to H287A DBD mutant or the R286A DBD mutant GFP-FOXM1 cell lines. The red dots represent peaks where FOXM1 binding is significantly increased/decreased compared to the WT