Fig. 2
From: FOXM1 binds directly to non-consensus sequences in the human genome
Generation of inducible GFP-FOXM1 expressing HEK293 cell lines. a Western blot showing induction of GFP-FOXM1B expression following addition of doxycycline for 24 h while levels of endogenous FOXM1 are unchanged. The blot was probed using antibodies for FOXM1 and ACTB. b Quantitative PCR (qPCR) showing RNA expression levels of total FOXM1 (GFP-FOXM1 and endogenous) and endogenous FOXM1 only (FOXM1-UTR). c qPCR of FOXM1 target genes transcript levels in GFP-FOXM1 cells treated ± doxycycline for 24 h and expressed relative to the levels in the parent HEK293 cells (Negative) for each transcript. Data show triplicate experiments ± SEM. d Western blot showing protein levels of GFP-FOXM1 in WT and mutant cell lines treated ± doxycycline (Dox) at 1 μg/mL for 24 h