Fig. 4

PCR-Tag analysis of a synIII segment. a The YCL061C.3 locus-specific PCR-Tag forward (F) and reverse (R) primers for the wild type (WT) and synIII are shown. The changes between the two are shaded. PCR-Tags are short pairs of recoded segments used as genetic markers to verify introduction of a synthetic sequence and removal of native sequence. Pairs of 25–28 bp sequences about 500 bp apart were recoded with synonymous codons such that >33 % of the bases were changed; the first and last base PCR-Tag primers were coded to be different between the WT and synIII sequences. b Agarose gel profiles of PCR-Tag analysis of a WT DNA segment and the corresponding synthetic synIII segment (YCL061C.3 to YCL050C.1). A virtual gel image was generated using LabChip GX software version 4.0.1418.0