Fig. 2From: Rewriting the blueprint of life by synthetic genomics and genome engineeringMultiplex automated genome engineering (MAGE) and conjugative assembly genome engineering (CAGE). a Use of MAGE (refer to [8] for more details) to replace all TAG codons with TAA in E. coli. b Use of CAGE (refer to [24] for more details) to incorporate a donor (D) into a recipient (R) genome. oriT in the donor genome serves as the transfer initiation pointBack to article page