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Fig. 5 | Genome Biology

Fig. 5

From: Drosophila Imp iCLIP identifies an RNA assemblage coordinating F-actin formation

Fig. 5

Reduced Imp levels result in aberrant neuronal patterning. a Theoretical genotypes and observed abnormal embryos in the F1 generation. FM7c designates an X-chromosome balancer containing a wild-type (wt) imp gene and β-gal gene, whereas imp G0072 contains a P-element in the imp locus on the X chromosome. Wild type and FM7c balanced wild type exhibited equal rates of abnormal embryos. A binominal P value of <0.001 was calculated for the imp G0072 mutant compared with either wild type or FM7c balanced wild type. Number of embryos counted: wt/wt + wt/Y cross 575 embryos, wt/FM7c + FM7c/Y cross 712 embryos, imp G0072/ FM7c + FM7c/Y cross 558 embryos. b Whole-mount immunohistochemistry with the monoclonal antibody 22C10 staining neurofilamental Futsch (red), and β-gal (green) marking the X-chromosome balancer. Top left: an embryo with multiple wild-type Imp alleles and a nervous system that appears normal (scale bar 50 μm). Bottom left: an embryo with one Imp allele which is morphologically abnormal and an aberrant nervous system (scale bar 50 μm). Top right: an imp G0072 hemizygous male embryo with a relatively normal morphology and a distinguishable central nervous system exhibiting pathfinding defects (scale bar 100 μm). Bottom right: an imp G0072 hemizygous abnormal male embryo revealing extensive patterning defects (scale bar 50 μm)

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