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Fig. 4 | Genome Biology

Fig. 4

From: Drosophila Imp iCLIP identifies an RNA assemblage coordinating F-actin formation

Fig. 4

Knockdown of Imp leads to diminished F-actin levels. a Structured illumination of Alexa 488-phalloidin (green) and Alexa 568 (red) Imp in Drosophila S2 cells. b Selected visual fields of untreated, luciferase dsRNA-treated, or imp dsRNA-treated S2 cells, in the presence or absence of the ROCK inhibitor Y-27632. c The absolute frequencies of Alexa 488 and Alexa 568 pixels in a visual field of untreated S2 cells and cells with Imp knock-down, which were used to derive the average mean intensities shown in panel (d). d The pixel intensities of ten frames from each cell dish were measured, and the boxplots display the average mean intensity of Imp immunoreactivity (left panel) and phalloidin staining (right panel). P values were calculated using Student’s t-test. *P ≤ 0.05, **P ≤ 0.01 and ****P ≤ 0.0001

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