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Fig. 3 | Genome Biology

Fig. 3

From: Drosophila Imp iCLIP identifies an RNA assemblage coordinating F-actin formation

Fig. 3

Imp RNPs contain transcripts associated with neural development and reproduction. a Enrichment of combined iCLIP and PAR-iCLIP clusters (red curve) and RIP-seq data (blue curve) across the Imp targetome. On the x-axis, the transcripts are ranked according to the enrichment of the individual transcript shown on the y-axis. The horizontal dotted line at y-value 1 indicates equal relative amounts of CLIP clusters or RIP-seq reads and RNA-seq levels. b Venn diagram of highly ranked transcripts derived from six individual experiments utilizing three distinct experimental approaches. In each experiment the 200 most highly ranked transcripts are identified, and the common pool for each experimental approach is represented by the three ellipses (157 for RIP-seq, 154 for iCLIP, and 168 for PAR-iCLIP) c GO term analyses of the 86 transcripts present among the 200 most highly ranked transcripts in each of six individual experiments utilizing three distinct experimental approaches and a P value cutoff of 0.01. Figure S3b in Additional file 1 lists the protein family or conserved domains encoded by each of the 86 Drosophila transcripts. d Standardized transcript profile showing the average number of motifs per nucleotide predicted with the hidden Markov model. The y-axis represents the average motif counts per nucleotide and the x-axis shows the position of the motif along the standardized transcript. The red line indicates the number of matches in the top 86 transcripts identified by iCLIP, PAR-iCLIP and RIP-seq datasets. The blue line displays the average motifs in all transcripts with a least 30 % RNA-seq coverage. Vertical dashed lines indicate borders between UTRs and CDS

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