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Fig. 5 | Genome Biology

Fig. 5

From: A versatile reporter system for CRISPR-mediated chromosomal rearrangements

Fig. 5

CRISPR/Cas9-mediated deletion and inversion of the Pten genomic region in mouse liver. a Schematic of deletion or inversion of a 50 kb Pten region on mouse chromosome 19. b Two pX330 plasmids expressing Cas9 and sgRNAs (sgPten.a + b) were co-delivered to mice via hydrodynamic injection. Red triangles indicate the sites recognized by sgPten.a and sgPten.b. The black arrow denotes the promoter. Liver tissue was analyzed 2 weeks later. c Quantification of Pten immunohistochemistry (n = 5 mice). Error bars are the s.d. d Pten-negative hepatocytes (arrows) were detected via immunohistochemistry. e, f A PCR reaction detected deletion (e) or inversion (f) of the targeted Pten region. g, h Deep sequencing of PCR bands detected approximately 14 % indel at the predicted deletion re-ligation site. We did not detect any indels with >1 % frequency at the predicted inversion repair site. Shown are representative IGV images of two biological replicates. i Quantification of deletion indels in (g)

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