Fig. 4
From: A versatile reporter system for CRISPR-mediated chromosomal rearrangements
iGFP inversion is LIG4-dependent in human cells. a HCT116 wildtype (WT) or HCT116 LIG4−/− cells were co-transfected with iGFP reporter and sgiGFP.1 + 2. Top: microscopic images. Bottom: FACS analysis of GFP positive cells. b Equal transfection efficiency in HCT116 WT and LIG4−/− cells transfected with tdTomato plasmid. c Quantification of GFP+ cells in (a). Error bars are the s.d. (n = 3)