Genetic analysis of ZFP57 consensus binding motif.
a Consensus ZFP57 binding motif identified using MEME algorithm analysis of DNA sequences underlying ChIP-seq peaks. b Distribution of genetically determined monoallelic Zfp57 peaks based on motif integrity on the unbound vs. bound alleles: SNPs disrupting all of the motifs (five sites, red), some of the motifs with at least one remaining intact (eight sites, orange) and none of the motifs (seven sites, green). No motifs could be found on either genetic alleles in two cases (grey). c Diagram indicating which bases within the consensus are varied between BL6 and Cast genomes in strain-specific peaks. Long bars, short bars and triangle indicate deletion, SNP and insertion events, respectively. d Putative mechanisms for ZFP57 allele-specific binding. Top: ZFP57 bound, motif intact and methylated. Bottom left: ZFP57 does not bind because motif is mutated. Bottom right: ZFP57 does not bind because motif is unmethylated in a given genetic background