Fig. 1
From: Visualizing translocation dynamics and nascent transcript errors in paused RNA polymerases in vivo
RNET-seq, the read-length-specific NET-seq approach for the analysis of transcriptional pausing and errors in vivo. (a) An overview of the preparation of the RNA samples for RNET-seq. The 3′ RNA transcripts protected by RNAP from RNases were isolated from E. coli cells. (b) Different distribution of read lengths between the WT and ΔgreAB cells