Interindividual epigenetic variation at VTRNA2-1.
(a) UCSC browser shot of the VTRNA2-1 region on chromosome 5. A cluster of five bins with high positive SIVI (top track) overlaps VTRNA2-1. Blueprint Epigenome DNA methylation data on monocytes from healthy individuals (orange) confirm interindividual variation in this same region. (b) Bisulfite pyrosequencing results for two individuals with discordant VTRNA2-1 methylation. T/C polymorphisms resulting from bisulfite conversion at three CpG sites are highlighted in gray. (c) Inter-tissue correlations of VTRNA2-1 methylation across kidney, liver, and brain of 17 Asian cadavers confirm systemic nature of interindividual variation. (d) Clonal bisulfite sequencing data on PBL DNA of two Gambian individuals (both A/A at SNP rs9327740) confirm pyrosequencing data and suggest interindividual variation in VTRNA2-1 methylation is not driven by local genetic variation. Columns and rows correspond to CpG sites and individual clones, respectively. Filled circles indicate methylation; gray circles indicate missing data.