Brain tumor is a sequence-specific RNA-binding protein that directs maternal mRNA clearance during the Drosophila maternal-to-zygotic transition

Table 6 Comparison of gene annotation enrichment analyses for BRAT-associated mRNAs and mRNAs upregulated in brat mutant embryos in Classes A, B, and C

	All BRAT-associated mRNAs	mRNAs associated only with BRAT	PUM and BRAT co-target mRNAs	mRNAs upregulated in *brat* mutant embryos
		mRNAs associated only with BRAT	PUM and BRAT co-target mRNAs	Class A	Class B	Class C
Biological and developmental
Embryonic pattern specification/Axis specification	+	.	+	.	.	+
Neuroblast fate determination	.	.	+	.	.	.
Cellular
Cell motility/cell migration	.	.	+	.	.	.
Molecular
Signal transduction	+	.	+++	.	.	+
Regulation of transcription	.	.	+	.	.	.
Protein kinase activity	+	.	.	.	.	+
Phosphatase activity	+	.	.	.	.	+
Integral membrane proteins	+++++	+++	+++	.	+	.
Ion transport/homeostasis	+++	++	+	.	+++	.
Phospholipid/phosphoinositide metabolism	+++	+++	.	.	+	.

+ FDR = 1% to 10%; ++ FDR = 0.1% to 1%; +++ FDR = 0.001% to 0.1%; ++++ FDR = 10⁻⁵% to 0.001%; +++++ FDR <10⁻⁵%; ‘.’ = not significant (FDR >10% and Benjamini P value >0.1). FDR values represent the most significantly enriched annotation term related to the function listed. Analyses were performed using the DAVID functional annotation tool [42,43]. For details see Additional files 8 and 16.

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