Figure 2

PUM- and BRAT-binding motifs. (A) Motif enriched among PUM-associated mRNAs, identified by de novo motif discovery. (B) Motif enriched among BRAT-associated mRNAs, identified by de novo motif discovery. (C) Comparison of the motif enriched among BRAT-associated mRNAs with the sequences of the ‘Box A’ motifs from the two hb NREs and the cycB NRE. Note that the Box A motif from hb NRE2 mediates repression by BRAT, but the Box A motifs from hb NRE1 and the cycB NRE do not (see text). (D) In vitro BRAT-binding motif determined by RNAcompete using purified GST-BRAT-NHL domain.