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Figure 7 | Genome Biology

Figure 7

From: Intra-tumor genetic heterogeneity and alternative driver genetic alterations in breast cancers with heterogeneous HER2 gene amplification

Figure 7

Signaling pathway activation of ER-positive MCF7 and T47D cell lines expressing wild-type (WT) or I767M mutant HER2. Whole-cell lysates of MCF7 and T47D, stably expressing empty vector control, wild-type HER2 or I767M mutant HER2 were analyzed by western blotting for total and phosphorylated levels of HER2, AKT, ERK1/2 and rpS6 on the same membrane, detected by near infrared two-color detection and quantified (LI-COR; Odyssey). Phospho-/total protein ratios are shown below. NRG1, neuregulin-1.

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Genome Biology

ISSN: 1474-760X

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