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Table 1 Generation of reporter knock-in mice by Cas9 mRNA or protein combined with sgRNA or crRNA/tracrRNA

From: Cloning-free CRISPR/Cas system facilitates functional cassette knock-in in mice

Cas9 Guide RNA Injected Transferred (%) Newborn (%) Targeted (%) Knock-in (%)
mRNA sgRNA 86 58 (67.4) 8 (13.8) 8 (100) 1 (12.5)
Protein sgRNA 135 112 (83.0) 19 (17.0) 5 (26.3) 0 (0)
Protein crRNA and tracrRNA 107 65 (60.7) 11 (16.9) 9 (81.8) 5 (45.5)
  1. Percentages were calculated using the number in each column as the numerator and the number in the column to its left as the denominator except for Knock-in. Percentages of Knock-in were calculated using the number in the Knock-in column as the numerator and the number in the Newborn column as the denominator. Note that the results of protein injections with high concentrations of guide RNAs are shown. All results are shown in Tables S2 and S3 in Additional file 1.