Cas9
|
Guide RNA
|
Injected
|
Transferred (%)
|
Newborn (%)
|
Targeted (%)
|
Knock-in (%)
|
---|
mRNA | sgRNA | 86 | 58 (67.4) | 8 (13.8) | 8 (100) | 1 (12.5) |
Protein | sgRNA | 135 | 112 (83.0) | 19 (17.0) | 5 (26.3) | 0 (0) |
Protein | crRNA and tracrRNA | 107 | 65 (60.7) | 11 (16.9) | 9 (81.8) | 5 (45.5) |
- Percentages were calculated using the number in each column as the numerator and the number in the column to its left as the denominator except for Knock-in. Percentages of Knock-in were calculated using the number in the Knock-in column as the numerator and the number in the Newborn column as the denominator. Note that the results of protein injections with high concentrations of guide RNAs are shown. All results are shown in Tables S2 and S3 in Additional file 1.