Evolutionary constraint network for patient EV007 with 8 tumor samples and 55 SSNVs. Each node is associated with the binary profile of its corresponding SSNV group, the VAF centroid vector, and the number of SSNVs assigned to it. The edges represent the potential precedence relationships between the node SSNVs. The spanning tree reported for patient EV007 is highlighted (see ‘Results and discussion’). GL, germ line; SSNV, somatic single nucleotide variant; VAF, variant allele frequency.