Figure 2

ChIP-PCR validation of H3K36me3 hypomethylation in sdg8-5 compared with WT. ChIP-PCR was performed to validate the ChIP-Seq results of genes hypomethylated with H3K36me3 in sdg8-5 compared with WT. (A) ChIP-PCR primers (blue columns) and H3K36me3 ChIP-Seq profiles (red plot) of these genes. (B) ChIP-PCR results confirmed the ChIP-Seq results. C, carbon; IP, immunoprecipitation; L, light. The error bars represent the standard error of the mean.