Figure 2

Comparison of methods for fecal sampling. (A,B) The samples collected in sub-studies 1 (d₇) and 2 (d₃₉₂) clustered by subject (A) and time point (B) but not according to the preservation method (frozen, RNALater (+4 to 10°C or RT for 1 week (1w) on d₇; frozen and RNALater +4°C, 24 h on d₃₉₂) applied (complete linkage clustering based on Euclidean distance). (C) Shannon diversity index, richness and evenness are shown for d₇ (upper panels) and d₃₉₂ (lower panels) and statistically significant differences are indicated by asterisks with P-value ≤0.05 (unpaired Wilcoxon test).