Sites under selection within the Apis, Bombus phylogeny for three genes of interest. The title for each gene presents the OrthoDB accession, the gene name, and the immune category. We only present a subset of the genes that showed an overall signature for selection highlighting codons at three different significance thresholds: Bayesian posterior probability >0.75 (plus signs along the top of each panel), >0.95 (x’s), and where Eω - sqrt(Var(ω)) > 1.25 (circles). The blue shadow indicates an estimate of error at each codon. We show Pfam domains in colored blocks and Phobius regions along the x-axis. Crosshatched regions were trimmed from the alignment.