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Table 4 Search for immediate and persistent changes in RNA transcript levels after ED exposure of G1 prospermatogonia

From: Deleterious effects of endocrine disruptors are corrected in the mammalian germline by epigenome reprogramming

     Cutoff 1.5-fold, P <0.05 Cutoff 1.05-fold, P <0.05
  Condition 1 Condition 2 Probes a Probes Unique Common G1R-G2R Probes Unique Common G1R-G2R
Female-Male G1R BPA F G1R BPA M 12,310       
  G1R DEHP F G1R DEHP M 11,886       
  G1R VZ F G1R VZ M 11,877       
  G1R OIL F G1R OIL M 11,848       
ED-OIL G1R BPA F G1R OIL F 0 230 14   1,592 933  
  G1R DEHP F G1R OIL F 0 217 50   2,284 1,590  
  G1R VZ F G1R OIL F 0 194 20   2,133 1,412  
  G1R BPA M G1R OIL M 0 247 125   2,552 1,842  
  G1R DEHP M G1R OIL M 7 292 48 1 (0/1) 5,210 3,639 325 (77/325)
  G1R VZ M G1R OIL M 2 549 61 8 (0/8) 6,499 4,295 284 (30/284)
  G2R DEHP M G2R OIL M 0 199 12 1 (0/1) 2,456 1,482 325 (77/325)
  G2R VZ M G2R OIL M 0 186 16 8 (0/8) 1,912 1,044 284 (30/284)
Average number of probes    264 43   3,080 2,029  
  1. aCutoff: 1.5-fold, FDR P <0.05.
  2. Prospermatogonia were purified at 17.5 dpc from female (F) or male (M) fetuses exposed in utero (G1R) to BPA, DEHP, VZ, or vehicle control (‘oil’). Prospermatogonia were also purified from the next generation (G2R). Trancription of mRNA was measured using Affymetrix 1.0ST chips. Transcription differences were detected between conditions 1 and 2. The hits are tabulated according the cutoff values, shown in the heading, as probes and unique transcripts. Bold numbers are higher than average among ED treatments for the given cutoff values. Common changes were detected between the unique transcripts that change in G1R and G2R samples for the same treatment; the numbers of those that changed in the same direction are in parentheses.