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Table 4 Search for immediate and persistent changes in RNA transcript levels after ED exposure of G1 prospermatogonia

From: Deleterious effects of endocrine disruptors are corrected in the mammalian germline by epigenome reprogramming

    

Cutoff 1.5-fold, P <0.05

Cutoff 1.05-fold, P <0.05

 

Condition 1

Condition 2

Probes a

Probes

Unique

Common G1R-G2R

Probes

Unique

Common G1R-G2R

Female-Male

G1R BPA F

G1R BPA M

12,310

      
 

G1R DEHP F

G1R DEHP M

11,886

      
 

G1R VZ F

G1R VZ M

11,877

      
 

G1R OIL F

G1R OIL M

11,848

      

ED-OIL

G1R BPA F

G1R OIL F

0

230

14

 

1,592

933

 
 

G1R DEHP F

G1R OIL F

0

217

50

 

2,284

1,590

 
 

G1R VZ F

G1R OIL F

0

194

20

 

2,133

1,412

 
 

G1R BPA M

G1R OIL M

0

247

125

 

2,552

1,842

 
 

G1R DEHP M

G1R OIL M

7

292

48

1 (0/1)

5,210

3,639

325 (77/325)

 

G1R VZ M

G1R OIL M

2

549

61

8 (0/8)

6,499

4,295

284 (30/284)

 

G2R DEHP M

G2R OIL M

0

199

12

1 (0/1)

2,456

1,482

325 (77/325)

 

G2R VZ M

G2R OIL M

0

186

16

8 (0/8)

1,912

1,044

284 (30/284)

Average number of probes

  

264

43

 

3,080

2,029

 
  1. aCutoff: 1.5-fold, FDR P <0.05.
  2. Prospermatogonia were purified at 17.5 dpc from female (F) or male (M) fetuses exposed in utero (G1R) to BPA, DEHP, VZ, or vehicle control (‘oil’). Prospermatogonia were also purified from the next generation (G2R). Trancription of mRNA was measured using Affymetrix 1.0ST chips. Transcription differences were detected between conditions 1 and 2. The hits are tabulated according the cutoff values, shown in the heading, as probes and unique transcripts. Bold numbers are higher than average among ED treatments for the given cutoff values. Common changes were detected between the unique transcripts that change in G1R and G2R samples for the same treatment; the numbers of those that changed in the same direction are in parentheses.
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Genome Biology

ISSN: 1474-760X

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