Table 2 Significant changes between control and ED-treated samples after disturbing G1R DMR establishment
% MAT allele | ||||||||
|---|---|---|---|---|---|---|---|---|
Organ | ED | Difference (%) | SD (%) | Student’s t -test P value | Imprinted base line | Oil | Treatment | |
G2 soma trough paternal germ line - DMR DNA methylation (Figure 3 C) | ||||||||
Peg1-Mest | Head | VZ | -9.1 | 2.523 | 0.038 | YES (MAT) | 95 | 86 |
Nespas | Embryo | VZ | -6.9 | 3.150 | 0.022 | YES (MAT) | 91 | 84 |
Slc38a4 (b) | Lung | VZ | -5.9 | 1.747 | 0.006 | YES (MAT) | 94 | 88 |
IG-DMR | Lung | VZ | 5.1 | 1.513 | 0.010 | YES (PAT) | 5 | 10 |
G2 soma through maternal germ line - DMR DNA methylation (Figure 3 D) | ||||||||
Peg10 | Placenta | VZ | -23.4 | 3.247 | 0.000 | YES (MAT) | 100 | 77 |
Peg10 | Head | VZ | -9.5 | 2.146 | 0.035 | YES (MAT) | 95 | 85 |
Nespas (a) | Yolk sac | VZ | -8.5 | 4.049 | 0.023 | YES (MAT) | 80 | 71 |
Snrpn | Placenta | VZ | -8.4 | 4.675 | 0.049 | YES (MAT) | 95 | 86 |
Snrpn | Head | VZ | -7.7 | 2.521 | 0.020 | YES (MAT) | 94 | 86 |
Zac1 (a) | Yolk sac | DEHP | -7.3 | 2.129 | 0.037 | YES (MAT) | 83 | 76 |
KvDMR1 | Head | BPA | -7.2 | 1.497 | 0.001 | YES (MAT) | 100 | 93 |
Peg3 | Head | VZ | -6.4 | 2.483 | 0.034 | YES (MAT) | 99 | 92 |
Zac1 (a) | Placenta | VZ | -5.9 | 3.248 | 0.036 | NO | 73 | 67 |
Zac1 (b) | Placenta | VZ | -5.7 | 2.239 | 0.018 | NO | 66 | 61 |
Peg3 | Yolk sac | VZ | -5.6 | 2.541 | 0.022 | YES (MAT) | 88 | 83 |
Zac1 (a) | Head | VZ | -5.5 | 1.528 | 0.036 | YES (MAT) | 85 | 79 |
Rasgrf1 | Head | VZ | 5.4 | 1.343 | 0.003 | YES (PAT) | 6 | 12 |
Rasgrf1 | Head | DEHP | 5.5 | 2.038 | 0.011 | YES (PAT) | 6 | 12 |
Rasgrf1 | Heart | DEHP | 6.4 | 1.413 | 0.003 | YES (PAT) | 15 | 22 |
IG-DMR (a) | Liver | VZ | 9.0 | 1.087 | 0.002 | YES (PAT) | 9 | 18 |
IG-DMR (a) | Head | VZ | 11.2 | 3.210 | 0.048 | YES (PAT) | 4 | 15 |
G2 soma through paternal germ line - RNA transcripts (Figure 4 ) | ||||||||
Nesp | Lung | DEHP | -14.3 | 2.166 | 0.001 | NO | 45 | 31 |
Nesp | Heart | DEHP | -11.9 | 3.157 | 0.042 | NO | 45 | 33 |
Slc22a3 | Head | VZ | -7.8 | 2.097 | 0.027 | NO | 53 | 46 |
Ascl2 | Liver | VZ | -7.1 | 3.323 | 0.043 | NO | 77 | 70 |
Cdkn1c | Embryo | BPA | -6.7 | 2.865 | 0.036 | YES (MAT) | 99 | 92 |
Slc22a3 | Liver | VZ | -6.0 | 2.261 | 0.011 | NO | 57 | 51 |
Slc22a3 | Liver | BPA | -6.0 | 2.925 | 0.025 | NO | 57 | 51 |
Cdkn1c | Embryo | VZ | -5.8 | 1.367 | 0.021 | YES (MAT) | 99 | 93 |
Cdkn1c | Embryo | DEHP | -5.8 | 0.535 | 0.014 | YES (MAT) | 99 | 93 |
Slc22a3 | Lung | BPA | -5.7 | 2.710 | 0.031 | NO | 64 | 58 |
Usp29 | Lung | BPA | 5.7 | 1.424 | 0.040 | YES (PAT) | 3 | 9 |
Gnasxl | Heart | VZ | 6.3 | 1.940 | 0.027 | NO | 26 | 32 |
Rasgrf1 | Heart | VZ | 9.0 | 0.722 | 0.006 | YES (PAT) | 18 | 27 |
Gnasxl | Lung | VZ | 15.3 | 3.644 | 0.002 | NO | 31 | 46 |
G2 soma through maternal germ line - RNA transcripts ( Additional file 3 ) | ||||||||
Nesp | Placenta | DEHP | -12.7 | 4.922 | 0.034 | YES (MAT) | 80 | 67 |
Slc22a3 | Yolk sac | DEHP | -11.0 | 0.620 | 0.030 | YES (MAT) | 97 | 86 |
Ascl2 | Liver | VZ | -9.4 | 1.324 | 0.034 | NO | 71 | 61 |
Gnasxl | Yolk sac | DEHP | -8.6 | 3.204 | 0.035 | NO | 52 | 43 |
G3 soma through paternal germ line (lung and heart) - RNA transcripts (Figure 5 B) | ||||||||
Cobl | Heart | BPA | -28.0 | 2.289 | 0.004 | NO | 79 | 51 |
Cobl | Heart | VZ | -27.9 | 0.640 | 0.001 | NO | 79 | 51 |
Cobl | Lung | VZ | -19.7 | 2.033 | 0.005 | NO | 70 | 51 |
Cobl | Lung | BPA | -19.4 | 2.729 | 0.018 | NO | 70 | 51 |
Phlda2 | Lung | VZ | -11.8 | 3.974 | 0.032 | NO | 79 | 68 |
Cobl | Heart | DEHP | -11.1 | 1.206 | 0.003 | NO | 79 | 68 |
Rtl1/Rtl1as | Lung | BPA | -10.0 | 1.649 | 0.021 | YES (MAT) | 90 | 80 |
Tnfrsf22 | Heart | VZ | -9.9 | 1.942 | 0.026 | NO | 58 | 48 |
Rtl1/Rtl1as | Lung | VZ | -8.7 | 4.378 | 0.007 | YES (MAT) | 90 | 81 |
Tnfrsf22 | Heart | BPA | -8.1 | 1.070 | 0.022 | NO | 58 | 50 |
Tnfrsf22 | Lung | BPA | -7.6 | 1.305 | 0.023 | NO | 57 | 49 |
Ascl2 | Heart | DEHP | -6.2 | 2.372 | 0.011 | NO | 54 | 48 |
Tnfrsf22 | Lung | VZ | -5.5 | 2.043 | 0.025 | NO | 57 | 51 |
Atp10a | Heart | VZ | 5.1 | 1.285 | 0.010 | NO | 48 | 53 |
Sfmbt2 | Lung | VZ | 6.5 | 0.690 | 0.003 | NO | 41 | 48 |
Zim1 | Lung | BPA | 6.7 | 1.473 | 0.010 | YES (MAT) | 90 | 97 |
Snrpn | Lung | VZ | 9.9 | 2.488 | 0.019 | YES (PAT) | 11 | 21 |
Slc22a3 | Lung | BPA | 10.0 | 7.898 | 0.017 | NO | 50 | 60 |
Nespas (a) | Lung | VZ | 10.3 | 2.375 | 0.036 | YES (PAT) | 3 | 13 |
Ascl2 | Lung | BPA | 12.7 | 3.520 | 0.000 | NO | 46 | 59 |
Asb4 | Heart | VZ | 27.2 | 8.101 | 0.019 | NO | 61 | 88 |
Asb4 | Heart | BPA | 27.2 | 2.591 | 0.015 | NO | 61 | 88 |