Selected top hits of transgenerationally inherited DNA methylation aberrations. Prospermatogonia of G1 fetuses were treated with ED or oil control in utero as depicted in Additional file 4. Next, DNA methylation was mapped using MIRA-chip and custom Nimblegen arrays in purified G1R and G2R prospermatogonia at 17.5 dpc in triplicate and in adult spermatozoa in duplicate. Immediate and persistent changes are tabulated in Table 3 and Additional file 8. (A) Selected top persistent hits are shown from the analysis in duplicates labeled at the top according to the comparisons in Table 3 and marked with arrowheads (up for increase and down for decrease) (B) A selected IAP-flank region where common changes were detected in MGC samples between G1R and G2R at and between G2R MGC and G2R sperm. (C) The H19-Igf2 imprinted DMR is shown as a positive control for the DNA methylation signal (black rectangle). DNA methylation signals of MIRA versus input DNA are plotted as -log10 P values ranging from 0 to 8.3 for experimental and control replicate samples. Note that these top changes are minor and not highly significant.