Figure 5

Knockdown of Firre or Ctcf decreases association of Firre and Dxz4 to the nucleolus. (A) Association frequency (in % of nuclei) between Firre or Dxz4 and the nucleolus as determined by DNA-FISH and immunostaining is significantly reduced after either Firre or Ctcf knockdown in Patski cells. At least 100 nuclei were scored in each experiment. P values were determined by two-tail unpaired student t-test. Error bars represent s.e.m. (B) Occupancy by CTCF and RAD21 at Firre and Dxz4 before and after knockdowns of Firre or Ctcf. ChIP enrichment is shown as the ratio between ChIP and input fractions as measured by qRT-PCR. Error bars represent s.e.m.