The effect of sequencing error on estimation accuracy. Unsigned error is defined as: |AĜS - AGS|/AGS. (A) MicrobeCensus was used to estimate the AGS of 20 metagenomes that were simulated with up to a 5% sequencing error rate. Metagenomes were composed of 100-bp reads from prokaryotes. (B) MicrobeCensus was used to estimate the AGS of 10 metagenomes that were composed of real Illumina reads pooled from 10 randomly chosen isolate sequencing projects.