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Figure 8 | Genome Biology

Figure 8

From: MUSiCC: a marker genes based framework for metagenomic normalization and accurate profiling of gene abundances in the microbiome

Figure 8

The impact of MUSiCC on pooling samples from multiple studies. (A) A principal Coordinate Analysis (PCoA) plot, illustrating the variation within the pathway-level functional profiles of healthy individuals from three independent studies of the human gut microbiome, using compositional normalization. Each dot represents a single sample and the proportion of variance explained by each of the first two principal coordinates is indicated on the axes’ labels. (B) A similar PCoA plot after the application of MUSiCC. (C) The number of T2D-associated pathways that are recovered (see Methods) when using as control the original T2D study control samples (left), HMP samples (middle), or IBD control samples (right). For each case, the number of pathways recovered with compositional normalization and with MUSiCC is illustrated.

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