Figure 1
Characterization of the transcriptional network controlled by FLC and SVP in leaves and apices. (A) Genes differentially expressed in svp-41 (pink), flc-3 (green) and svp-41 flc-3 (light-blue) loss of function mutants compared to wild-type SVP FLC FRI in leaves and apices. Plants were grown for 2 weeks under SD conditions, and leaves and apices were collected 8 h after dawn (Zeitgeber 8 (ZT8)). Only genes with fold-change above 2 and q-value <0.01 were selected as differentially expressed. (B) Transcriptional profile comparisons from (A) represented as a heatmap to highlight up-regulated (yellow) and down-regulated (blue) genes. Expression change is represented in log2 scale. (C) Schema of the transcriptional network under study. (D) Statistical analysis of signalling allocation from identified clusters. A, B, C, D and A’, B’, C’, D’ denote the different expression clusters found in leaves and apices, respectively. Asterisks denote P-value <0.01. (E) Average change in expression level relative to wild-type for genes located in the four clusters represented in (D). For each cluster, the average expression level in each mutant genotype is represented. Colour code is as in (A). Cluster A, 33 genes; cluster B, 29 genes; cluster C, 18 genes; cluster D, 62 genes; cluster A’, 8 genes; cluster B’, 11 genes; cluster C’, 5 genes; cluster D’, 6 genes. Mean values are accompanied by standard error.