Natural polymorphic variation in ChrY influences cytokine profiles of activated CD4
T cells from female offspring of B6-ChrY
consomic mice. Purified CD4+ T cells from B6-ChrYSJL and B6 lymph nodes were incubated with anti-CD3 and anti-CD28 monoclonal antibodies and the supernatants were tested for IFN-γ, IL-6, and IL-17 production after 24, 48, and 72 hours by ELISA. Data are representative of three independent experiments. IL-17 was below the limit of detection. Significance determined by two-way ANOVA followed by Holm-Sidak’s multiple comparisons test. *P ≤ 0.05; ***P ≤ 0.001; ****P ≤ 0.0001.