Figure 1
Polymorphic variation in ChrY influences susceptibility to EAE in female offspring. (A) Female offspring from B6-ChrY consomic strains were immunized with MOG35-55 using the 2× protocol and the clinical score was monitored over 30 days. The consomic strain represented by each line is color coded with the bar graph in (B). Wild-type B6 females are represented by black circles. The significance of the differences in disease course among the strains was determined by two-way ANOVA (interaction (F = 1.517; DFn = 406; DFd = 10,940; P < 0.0001), day post-injection (F = 176.1; DFn = 29; DFd = 10,940; P < 0.0001), and strain (F = 33.27; DFn = 14; DFd = 10,940; P < 0.0001)), followed by Holm-Sidak’s multiple comparisons against B6 (****P ≤ 0.0001). (B) The CDS was determined for each strain and the significance of the observed differences determined by one-way ANOVA followed by Holm-Sidak’s multiple comparisons against B6 (*P ≤ 0.05, **P ≤ 0.01; ****P ≤ 0.0001). The x-axis indicates the strain origin of ChrY. The dashed line indicates the CDS for B6. Significance of differences determined by one-way ANOVA followed by Fisher’s LSD test. Data represented as mean ± standard error of the mean. For (A,B), a heterogeneity test was used between cohorts of mice with no significant differences detected. Thus, data were pooled from three independent experiments and the total number of animals analyzed are included in (A). (C) Histopathology of the spinal cord from female offspring from B6-ChrY consomic strains immunized using the 2× protocol. Significance of observed differences was determined by one-way ANOVA followed by Holm-Sidak’s multiple comparisons test. n ≥ 15; *P ≤ 0.05, **P ≤ 0.01. Data represented as mean ± standard error of the mean.