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Figure 7 | Genome Biology

Figure 7

From: Modulation of microRNA editing, expression and processing by ADAR2 deaminase in glioblastoma

Figure 7

miRNA maturation of the wild-type and the edited versions of pri-miR-221, pri-miR-222 and pri-miR-21. (a) Left: the pri-miR-221 sequence structure, indicating the mutagenized/edited positions. Right: the mature miR-221 and -221* levels were measured by qRT-PCR in untreated HeLa cells (untr, black) and in HeLa cells transfected with wild-type pri-miR-221 (221 WT, dark gray), fully edited pri-miR-221 (221 ED, light gray) or pri-miR-221 edited at specific sites (221 ED +64; 221 ED -1,+1). (b) Left: the pri-miR-222 sequence structure, indicating the mutagenized/edited positions. Right: the mature miR-222 and -222* levels were measured by qRT-PCR in untreated HeLa cells (untr, black) and in HeLa cells transfected with wild-type pri-miR-222 (222 WT, dark gray), fully edited pri-miR-222 (222 ED, light gray) or pri-miR-222 edited at specific sites (222 ED +53; 222 ED -4; 222 ED -21; 222 ED -4,-21). (c) Left: the pri-miR-21 sequence structure, indicating the mutagenized/edited positions. Right: the mature miR-21 levels were measured by qRT-PCR in untreated HeLa cells (untr, black) and in HeLa cells transfected with wild-type pri-miR-21 (21 WT, dark gray) or edited pri-miR-21 (at sites +16, +46, +51) (21 ED, light gray). Mature miRNAs were normalized using RNU6B. The expression levels were calculated as a relative-fold increase compared with the untreated cells and arbitrarily set to 1. Mean ± standard error of the mean (n = 3), **P < 0.01, *P < 0.05 when each sample is compared with the corresponding wild-type pri-miR.

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