Figure 5

Modulation of miR-221, miR-222 and miR-21 and their precursors in HEK293T cell lines upon ADAR2 and ADAR2 E/A expression. (a) Expression levels, using qRT-PCR, of mature miR-221 (left panel), miR-222 (middle panel) and miR-21 (right panel) in HEK293T cells transiently co-transfected with either pri-miR-222/221 or pri-miR-21 plasmids and with ADAR2 or ADAR2 E/A. (b) In the same cells, the pri-miR-221 (left panel), pri-miR-222 (middle panel) and pri-miR-21 (right panel) expression levels measured by qRT-PCR are shown. Mature miRNAs and pri-miRNAs were normalized using RNU6B and GAPDH, respectively. The expression levels were calculated as a relative-fold increase compared with the sample transfected with the pri-miR-222/221 or pri-miR-21 plasmid arbitrarily set to 1. Mean ± standard error of the mean (n = 3), **P < 0.01, *P < 0.05.