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Figure 2 | Genome Biology

Figure 2

From: Modulation of microRNA editing, expression and processing by ADAR2 deaminase in glioblastoma

Figure 2

ADAR2 silencing in U118 cell line increases miR-221, miR-222 and miR-21 expression levels. (a) Left: qRT-PCRs of ADAR2 in U118 ADAR2 (ctrl, dark gray) and the same cell line stably transfected with siADAR2 (siADAR2, medium gray) or scramble (scr, light gray) plasmids. Each sample was normalized to GAPDH mRNA levels. Mean ± standard error of the mean (s.e.m.) (n = 3), **P < 0.01. Right: a representative immunoblot of ADAR2 in the cell lines analyzed. (b) Mature miR-221 (left panel), miR-222 (middle panel) and miR-21 (right panel) expression levels in U118 ADAR2, siADAR2 and scramble cell lines are shown. Each sample was normalized to RNU6B levels. Mean ± s.e.m. (n = 3), **P < 0.01, *P < 0.05. The expression levels were calculated as a relative-fold increase compared with U118 ADAR2 (ctrl) arbitrarily set to 1.

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