genome from DNA-seq data. The performance of Rockhopper 2 as well as two other assemblers, SOAPdenovo2 and Trinity, on three biological replicate DNA-seq experiments from E. coli is illustrated. (A) Specificity is the percentage of assembled contigs that align to the E. coli genome. (B) Sensitivity is the percentage of the E. coli genome sequence that is covered by assembled contigs aligning to the genome. (C) Execution time is the number of minutes that an assembler requires to execute on the DNA-seq data.